AboutInVivoMAbanti-mouseCLEC9A(CD370)
The7H11monoclonalantibodyreactswithmouseCLEC9A(C-typelectindomainfamilymember9A).CLEC9A,alsoknownasDNGR1(dendriticcellnaturalkillerlectingroupreceptor1)andCD370,isatypeIItransmembraneglycoproteinwithasingleextracellularC-typelectindomain.DNGR-1isrestrictedinitsexpression,beingfoundonlyonCD8α+,CD103+,CD11b−subsetsofDCsandplasmacytoidDCs.CLEC9Areportedlyfunctionsasanendocyticreceptorfornecroticcells.Itcanmediatethecross-presentationofdead-cellassociatedantigensbydendriticcellsinaSyk-dependentmanner.IthasbeenshownthattargetingantigentoDNGR-1onDC’sviacouplingantigentothe7H11antibodycanresultinactivationofantigenspecificCD8+Tcellresponsesinvivo.
InVivoMAbanti-mouseCLEC9A(CD370)Specifications
| Isotype | RatIgG1,κ |
| RecommendedIsotypeControl(s) | InVivoMAbratIgG1isotypecontrol,anti-horserADIshperoxidase(BE0088) |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer(IP0070) |
| Immunogen | RBL-2H3cellsexpressingmouseCLEC9AfusedtoanHAepitope |
| ReportedApplications | invivoAgtargetingtoCLEC9A+DCs Westernblot Immunoprecipitation Immunofluorescence Flowcytometry |
| Endotoxin |
|
| Purity |
|
| Formulation |
|
| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Theantibodysolutionshouldbestoredundilutedat4°C,andprotectedfromprolongedexposuretolight.Donotfreeze. |
| RRID | AB_2721034 |
