AboutInVivoMAbanti-mousePD-L1(B7-H1)
The10F.9G2monoclonalantibodyreactswithmousePD-L1(programmeddeathligand1)alsoknownasB7-H1orCD274.PD-L1isa40kDatypeItransmembraneproteinthatbelongstotheB7familyoftheIgsuperfamily.PD-L1isexpressedonTlymphocytes,Blymphocytes,NKcells,dendriticcells,aswellasIFNγstimulatedmonocytes,epithelialcellsandendothelialcells.PD-L1bindstoitsreceptor,PD-1,foundonCD4andCD8thymocytesaswellasactivatedTandBlymphocytesandmyeloidcells.EngagementofPD-L1withPD-1leadstoinhibitionofTCR-mediatedTcellproliferationandcytokineproduction.PD-L1isthoughttoplayanimportantroleintumorimmuneevasion.InducedPD-L1expressioniscommoninmanytumorsandresultsinincreasedresistanceoftumorcellstoCD8Tcellmediatedlysis.Inmousemodelsofmelanoma,tumorgrowthcanbetransientlyarrestedviatreatmentwithantibodieswhichblocktheinteractionbetweenPD-L1andPD-1.The10F.9G2antibodyhasbeenshowntoblocktheinteractionbetweenPD-L1andPD-1andbetweenPD-L1andB7-1(CD80).
InVivoMAbanti-mousePD-L1(B7-H1)Specifications
| Isotype | RatIgG2b, κ |
| RecommendedIsotypeControl(s) | InVivoMAbratIgG2bisotypecontrol,anti-keyholelimpethemocyanin(BE0090) |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH6.5DilutionBuffer(IP0065) |
| Immunogen | MouseCD274 |
| ReportedApplications |
|
| Endotoxin |
|
| Purity |
|
| Formulation |
|
| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Theantibodysolutionshouldbestoredundilutedat4°C,andprotectedfromprolongedexposuretolight.Donotfreeze. |
| RRID | AB_10949073 |
