AboutInVivoMAbanti-mouseICOSL(CD275)
TheHK5.3monoclonalantibodyreactswithmouseICOSL(inducIBLeTcellco-stimulatorligand)alsoknownasCD275,B7RP-1,andB7-H2.ICOSLisa40kDaimmunecheckpointproteinbelongingtotheIgreceptorsuperfamily.UponICOSLbinding,ICOSsignalingco-stimulatesTandBcellresponses.TheligandIsexpressedonantigenpresentingcellsincludingsplenicBcells,dendriticcells,andmacrophages.ICOSsignalingisalsothoughttobeimportantformaintainingregulatoryTcellhomeostasis.TheHK5.3antibodyhasbeenshowntoblockthebindingofICOSLtoICOSbothinvitroandinvivo.HK5.3treatmentofmicehasbeenreportedtoleadtoalossofregulatoryTcells.
InVivoMAbanti-mouseICOSL(CD275)Specifications
| Isotype | RatIgG2a, κ |
| RecommendedIsotypeControl(s) | InVivoMAbratIgG2aisotypecontrol,anti-trinitrophenol(BE0089) |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer(IP0070) |
| Immunogen | MouseB7-RP1transfectedcellline |
| ReportedApplications | invivoICOSLneutralization |
| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Theantibodysolutionshouldbestoredundilutedat4°C,andprotectedfromprolongedexposuretolight.Donotfreeze. |
| RRID | AB_1107566 |
