AboutInVivoPlusanti-mouseTIM-3(CD366)
TheRMT3-23monoclonalantibodyreactswithmouseTIM-3(Tcellimmunoglobulinandmucindomain-3)alsoknownasCD366.TIM-3isa60kDamemberoftheTIMfamilyofimmunecheckpointreceptorsandexistsasatypeItransmembraneglycoproteinwithamucin-likedomaininitsextracellularportionandatyrosinephosphorylationmotifinitscytoplasmicportion.TIM-3isspecificallyexpressedathighlevelsonthesurfaceofTh1lymphocyteswhereasTh2lymphocytesexpressTIM-1andTIM-2.TIM-3activationoccursviabindingtothecell-associatedC-typelectingalectin-9.UponbindingTIM-3inducesapoptosisofTh1cells.InhibitionofTIM-3signalinginmicehasbeenshowntoexacerbateexperimentalautoimmuneencephalomyelitis,promoteIFNγproductionandTh1cellproliferation.Tim-3hasalsobeenshowntoberequiredfortheinductionoftolerance,asbothTIM-3knockoutanimalsandmicetreatedwithTIM-3-Igfusionproteindisplaydefectsintheinductionofantigen-specifictolerance.Additionally,TIM-3signalingiscurrentlybeingexploredasacancerimmunotherapytargetasCD8TcellswhichexpressbothTIM-3andPD-1exhibitgreaterdefectsinbothcell-cycleprogressionandeffectorcytokineproductionthancellsthatexpressPD-1alone.TheRMT3-23antibodyactsasaTIM-3receptorantagoNISTandhasbeenshowntohavefunctionalactivityincludingsuppressingtumorcellgrowthinamurinesarcomamodel.
InVivoPlusanti-mouseTIM-3(CD366)Specifications
| Isotype | RatIgG2a, κ |
| RecommendedIsotypeControl(s) | InVivoPlusratIgG2aisotypecontrol,anti-trinitrophenol(BP0089) |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer(IP0070) |
| Immunogen | RecombinantmouseTIM-3 |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Aggregation |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Theantibodysolutionshouldbestoredundilutedat4°C,andprotectedfromprolongedexposuretolight.Donotfreeze. |
| MurinePathogenTestResults |
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| RRID | AB_10949464 |
