AboutInVivoMAbanti-mouseTNFα
TheXT3.11monoclonalantibodyreactswithmouseTNFα(tumornecrosisfactor-alpha)amultifunctionalproinflammatorycytokine.TNFαexistsasasoluble17kDamonomer,whichformshomotrimersincirculationorasa26kDamembrane-boundform.TNFαbelongstotheTNFsuperfamilyofcytokinesandsignalsthroughitstworeceptors,TNFR1andTNFR2whichcanbeactivatedbyboththesolubletrimericandmembrane-boundandformsofTNFα.TNFαisprimarilyproducedbymacrophagesinresponsetoforeignantigenssuchasbacteria(lipopolysaccharides),viruses,andparasitesaswellasmitogensandothercytokinesbutcanalsobeexpressedbymonocytes,neutrophils,NKcells,CD4Tcellsandsomespecializeddendriticcells.TNFαisknowntoplaykeyrolesinawidespectrumofBIOLOGicalprocessesincludingimmunoregulation,cellproliferation,differentiation,apoptosis,antitumoractivity,inflammation,anorexia,cachexia,septicshock,hematopoiesis,andviralreplication.TNFαdysregulationhasbeenimplicatedinavarietyofdiseases,includingautoimmunediseases,insulinresistance,andcancer.MouseandhumanTNFαshare79%aminoacidsequenceidentityhowever,mouseTNFαisglycosylatedwhilehumanTNFαisnot.TNFαknockoutanimalsdisplaydefectsinresponsetobacterialinfection,characterizedbydefectsinformingorganizedfolliculardendriticcellnetworksandgerminalcenterswithalackofprimaryBcellfollicles.
InVivoMAbanti-mouseTNFαSpecifications
| Isotype | RatIgG1 |
| RecommendedIsotypeControl(s) | InVivoMAbratIgG1isotypecontrol,anti-horserADIshperoxidase(BE0088) |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH8.0DilutionBuffer(IP0080) |
| Immunogen | RecombinantmouseTNFα |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Theantibodysolutionshouldbestoredundilutedat4°C,andprotectedfromprolongedexposuretolight.Donotfreeze. |
| RRID | AB_1107764 |
