AboutInVivoMAbanti-mouseTIM-3(CD366)
TheB8.2C12monoclonalantibodyreactswithmouseTIM-3(Tcellimmunoglobulinandmucindomain-3)alsoknownasCD366.ThisantibodybindstotheBALB/calleleofTIM-3whilereactivitytotheC57Bl/6alleleissignificantlyweaker.TIM-3isa60kDamemberoftheTIMfamilyofimmunecheckpointreceptorsandexistsasatypeItransmembraneglycoproteinwithamucin-likedomaininitsextracellularportionandatyrosinephosphorylationmotifinitscytoplasmicportion.TIM-3isspecificallyexpressedathighlevelsonthesurfaceofTh1lymphocyteswhereasTh2lymphocytesexpressTIM-1andTIM-2.TIM-3activationoccursviabindingtothecell-associatedC-typelectingalectin-9.UponbindingTIM-3inducesapoptosisofTh1cells.InhibitionofTIM-3signalinginmicehasbeenshowntoexacerbateexperimentalautoimmuneencephalomyelitis,promoteIFNγproductionandTh1cellproliferation.Tim-3hasalsobeenshowntoberequiredfortheinductionoftolerance,asbothTIM-3knockoutanimalsandmicetreatedwithTIM-3-Igfusionproteindisplaydefectsintheinductionofantigen-specifictolerance.Additionally,TIM-3signalingiscurrentlybeingexploredasacancerimmunotherapytargetasCD8TcellswhichexpressbothTIM-3andPD-1exhibitgreaterdefectsinbothcell-cycleprogressionandeffectorcytokineproductionthancellsthatexpressPD-1alone.
InVivoMAbanti-mouseTIM-3(CD366)Specifications
| Isotype | RatIgG1, κ |
| RecommendedIsotypeControl(s) | InVivoMAbratIgG1isotypecontrol,anti-horserADIshperoxidase(BE0088) |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer(IP0070) |
| Immunogen | MouseTim-3protein/Freundadjuvant |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Theantibodysolutionshouldbestoredundilutedat4°C,andprotectedfromprolongedexposuretolight.Donotfreeze. |
| RRID | AB_2687798 |
