The145-2C11monoclonalantibodyreactswithmouseCD3ε,a20kDatransmembranecell-surfaceproteinthatbelongstotheimmunoglobulinsuperfamily.CD3εisoneoffivepolypeptidechainsthatcombinetoformtheTCRcomplex.CD3εisexpressedonTlymphocytes,NK-Tcells,andtovaryingdegreesondevelopingthymocytes.CD3playsrolesinTCRsignaling,Tlymphocyteactivation,andantigenrecognition.The145-2C11antibodyhasbeenshowntoinduceTlymphocyteactivation,proliferation,andapoptosisinvitroviabindingandstimulatingtheTCR.WhenusedinvivotheantibodyisreportedtoproduceTcellactivation,anergy,ordeath.ActivationofrestingTcellsinvivoresultsincytokinereleaseandsubsequenttoxicitycausedbyAb-mediatedcross-linkingofTcellsandFcγreceptor(FcR)-bearingcells.Forthisreason,thenon-FcR-binding145-2C11f(ab")2fragmentsaremorecommonlyusedforinvivoapplications. The145-2C11antibodyhasbeenreportedtoblockthebindingofthe17A2antibodytoCD3ε+Tlymphocytes.
| Isotype | ArmenianHamsterIgG1 |
| RecomendedIsotypeControl(s) | InVivoMAbpolyclonalArmenianhamsterIgG |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | MouseBM10-37cytotoxicTcells |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinA |
| Storage | Undilutedat4°Cinthedark |
