The 7D4 monoclonal antibody reacts with mouse IL-2Rα also known as CD25, Ly-43, p55, or Tac. IL-2Rα is the 55 kDa ligand-binding subunit of the interleukin 2 receptor alpha chain. IL-2Rα is expressed on activated mature T and B lymphocytes, thymocyte subsets, pre-B cells, and T regulatory cells. IL-2Rα has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Alone, the IL-2Rα binds IL-2 with relatively low affinity however, when IL-2Rα associates with IL-2Rβ (CD122) and the common gamma chain (CD132) the complex binds IL-2 with high affinity. The 7D4 antibody recognizes a different epitope on CD25 than the PC-61.5.3 clone.
| Isotype | Rat IgM, κ |
| Recomended Isotype Control(s) | InVivoMAb polyclonal rat IgG |
| Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | IL-2-dependent BALB/c mouse T cell clone HT2 |
| Reported Applications | in vivo regulatory T cell inactivation |
| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2 μM filtered |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | T-Gel |
| Storage | Undiluted at 4°C in the dark |
