TheHB220antibodyreactswithmouseCD45RBa220kDatransmembraneproteintyrosinephosphataseexpressedonperipheralBcellsnaëâveTcellsthymocytesandweaklyonmacrophagesanddendriticcells.CD45RBisoneof5isoformsofCD45whichresultfromalternativesplicingofexons4-6.CD45RBresultsfromexon5splicing.CD45RBiscriticalforintracellularsignalinginTcellsinresponsetoantigenstimulationasindicatedbythesevereimmunodeficiencyobservedinCD45-deficientmice.AsTcellsprogressfromnaëâvetomemorycellsandbecomeactivatedCD45RBexpressionisdownregulated.AdditionallytheintensityofCD45RBexpressioncanbeusedtodifferentiatebetweenfunctionallydistinctCD4+Tcellsubsetswhichsecretedifferingcytokineprofiles.TheprimaryligandsforCD45includegalectin-1CD2CD3CD4andThy-1.
| Isotype | RatIgG2a |
| RecomendedIsotypeControl(s) | InVivoMAbmouseIgG1isotypecontrol,unknownspecificity |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | Anti-immunoglobulinactivatedmouseBcells |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |
