The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 also known as Intercellular Adhesion Molecule-1 (ICAM-1). CD54 is a 90 kDa single-chain transmembrane glycoprotein and a member of the Ig superfamily. CD54 is typically expressed on non-hematopoietic cells such as endothelial cells, thymic epithelial cells, and fibroblasts but can also be expressed on macrophages, T-lymphoblasts, germinal center B cells and dendritic cells. CD54 is a ligand for LFA-1, a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via CD54/LFA-1 to facilitate transmigration into tissues. Pro-inflammatory cytokines such as IL-1, TNF and IFNγ induce CD54 expression on endothelial cells and fibroblasts. The YN1/1.7.4 antibody has been shown to neutralize CD54 in vivo and inhibit CXCL1, IFNγ, and IL-17 production and neutrophil infiltration into inflammatory lesions.
| Isotype | Rat IgG2b, κ |
| Recomended Isotype Control(s) | InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin |
| Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | NS-1 mouse myeloma cells |
| Reported Applications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2 μM filtered |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein G |
| Storage | Undiluted at 4°C in the dark |
