TheOX-86monoclonalantibodyreactswithmouseOX-40alsoknownasCD134.OX-40isa50kDatypeImembraneglycoproteinandamemberoftheTNFreceptorsuperfamily.OX-40isexpressedonactivatedCD4andCD8Tcells,butisnotfoundonrestingnaïveTcellsormostrestingmemoryTcells.AlthoughitwasoriginallythoughtthatOX-40expressionwasrestrictedtoactivatedconventionalTcells,ithasnowbeenvisualizedonactivatedregulatoryTcells,NKTcells,NKcells,andneutrophils.OX-40playsamajorroleinregulatingbothCD4andCD8Tcellclonalexpansion.Itprovidesacostimulatorysignaltoanantigen-reactingnaiveTcellstoprolongproliferation,aswellasaugmenttheproductionofseveralcytokines.ThisisdemonstratedbyOX-40knockoutmicewhichgeneratefewerprimaryeffectorCD4Tcellsafterimmunization.FurThermore,invivotreatmentwithanagoNISTantibodytoOX-40hasbeenshowntostronglyenhancethegenerationofantigen-specificeffectorTcellsandpreventtheinductionofTcelltolerance.TheOX-86antibodyisanagonisticantibodythathasbeenshowntodelaytumorgrowthinvivo.
| Isotype | RatIgG1, κ |
| RecomendedIsotypeControl(s) | InVivoMAbratIgG1isotypecontrol,anti-horserADIshperoxidase InVivoMAbratIgG1Isotypecontrol,anti-trinitrophenol |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | RecombinantmouseOX40-CD4chimericprotein |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |
