TheJES5-2A5monoclonalantibodyreactswithmouseIL-10.IL-10isa17-21kDacytokinewhichisprimarilyproducedbymonocytesand,toalesserextent,Th2cells,mastcells,Tregulatorycells,andcertainsubsetsofactivatedTandBcells.IL-10haspleiotropiceffectsinimmunoregulationandinflammation.Itdown-regulatestheexpressionofTh1cytokines,MHCclassIIantigens,andcostimulatorymoleculesonmacrophages.ItalsoenhancesBcellsurvival,proliferation,andantibodyproduction.ThiscytokinecanblockNF-κBactivity,andisinvolvedintheregulationoftheJAK-STATsignalingpathway.Knockoutstudiesinmicesuggestthatthiscytokineisanessentialimmunoregulatorintheintestinaltract.TheJES5-2A5antibodyhasbeenshowntoneutralizethebioactivityofnaturalorrecombinantIL-10.
| Isotype | RatIgG1, κ |
| RecomendedIsotypeControl(s) | InVivoMAbratIgG1isotypecontrol,anti-horserADIshperoxidase InVivoMAbratIgG1Isotypecontrol,anti-trinitrophenol |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | RecombinantmouseIL-10 |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |
