TheR4-6A2monoclonalantibodyreactswithmouseIFNγ(interferongamma)a20kDasolublepleiotropiccytokineandthesolememberofthetypeIIclassofinterferons.IFNγisprimarilyproducedbyactivatedlymphocytesincludingT,B,NKcells,andILCs.IFNγexertsimmunoregulatory,anti-proliferative,anti-viral,andproinflammatoryactivitiesandplaysanimportantroleinactivation,growth,anddifferentiationofTandBlymphocytes,macrophages,NKcellsandothernon-hematopoieticcelltypes.Additionally,IFNγinducestheproductionofcytokines,Fcreceptor,andadhesionmoleculesandup-regulatesMHCclassIandIIantigenexpressionbyantigenpresentingcellsduringanimmuneresponse.IFNγhasalsobeenshowntomodulatemacrophageeffectorfunctions,influenceisotypeswitchingandinducethesecretionofimmunoglobulinsbyBcells.IFNγsignalsthroughtheIFNgammareceptorwhichexistsasaheterodimercomposedofCD119(IFNγreceptor1)andAF-1(IFNγreceptor2).TheIFNγreceptorisexpressedubiquitouslyonalmostallcelltypeswiththeexceptionofmatureerythrocytes.TheR4-6A2antibodyhasbeenshowntoneutralizebothnaturalandrecombinantIFNγ.
| Isotype | RatIgG1, κ |
| RecomendedIsotypeControl(s) | InVivoMAbratIgG1isotypecontrol,anti-horserADIshperoxidase InVivoMAbratIgG1Isotypecontrol,anti-trinitrophenol |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH8.0DilutionBuffer |
| Immunogen | Partially-purifiednativemouseIFNγ |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |
