The1D3monoclonalantibodyreactswithmouseCD19,aBcell-specific95kDatransmembraneglycoproteinoftheimmunoglobulinsuperfamily.CD19containstwoextracellularimmunoglobulin-likedomainsandanextensivecytoplasmictail.ItfunctionsasapositiveregulatorofB-cellreceptorsignalinginconjunctionwithCD21andCD81.CD19ishighlyexpressedinmostlymphomasandleukemiasincludingsomeearlyB-cellmalignanciesthatdonotexpressCD20.ForthesereasonsCD19isquicklybecominganattractivealternativetargetfortheimmunotherapyoflymphoproliferativedisorders.The1D3antibodyhasbeenreportedtodepleteBcellshowever,Bcelldepletionoftenrequirestreatmentwithacombinationof1D3,anti-mouseB220(clone RA3.3A1/6.1),anti-mouseCD22(clone Cy34.1),andanti-ratκlightchain(clone MAR18.5)antibodies.Pleaseseetheprovidedreferencesforprotocoldetails.
| Isotype | RatIgG2a, κ |
| RecomendedIsotypeControl(s) | InVivoMAbratIgG2aisotypecontrol,anti-trinitrophenol |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | K562cellsexpressingtheextracellulardomainofmouseCD19 |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |
