The F23.1 monoclonal antibody reacts with the Vβ 8.1, Vβ 8.2, and Vβ 8.3 TCR (V beta 8.1-3 T cell receptors) of mice having the b haplotype of the Tcrb gene complex (e.g., AKR, BALB/c, C3H, C57BL, DBA/1, DBA/2). The TCR is expressed on the surface of T lymphocytes and is responsible for recognizing fragments of antigen as peptides bound to MHC molecules. When the TCR engages with antigenic peptide and MHC the T lymphocyte is activated through signal transduction. The F23.1 antibody has been shown to activate Vβ 8 TCR-expressing T lymphocytes, as well as block cytolysis mediated by Vβ 8 TCR-expressing cytotoxic T lymphocytes. Additionally, in vivo treatment of neonatal mice with the F23.1 antibody can arrest intrathymic maturation of Vβ 8 TCR-expressing T cells.
| Isotype | Mouse IgG2a, κ |
| Recomended Isotype Control(s) | InVivoMAb mouse IgG2a isotype control, unknown specificity |
| Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | BALB.B Mouse lymph node and spleen T cells |
| Reported Applications | Flow cytometry |
| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2 μM filtered |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein G |
| Storage | Undiluted at 4°C in the dark |
