TheF23.1monoclonalantibodyreactswiththeVβ8.1,Vβ8.2,andVβ8.3TCR(Vbeta8.1-3Tcellreceptors)ofmicehavingthebhaplotypeoftheTcrbgenecomplex(e.g.,AKR,BALB/c,C3H,C57BL,DBA/1,DBA/2).TheTCRisexpressedonthesurfaceofTlymphocytesandisresponsIBLeforrecognizingfragmentsofantigenaspeptidesboundtoMHCmolecules.WhentheTCRengageswithantigenicpeptideandMHCtheTlymphocyteisactivatedthroughsignaltransduction.TheF23.1antibodyhasbeenshowntoactivateVβ8TCR-expressingTlymphocytes,aswellasblockcytolysismediatedbyVβ8TCR-expressingcytotoxicTlymphocytes.Additionally,invivotreatmentofneonatalmicewiththeF23.1antibodycanarrestintrathymicmaturationofVβ8TCR-expressingTcells.
| Isotype | MouseIgG2a, κ |
| RecomendedIsotypeControl(s) | InVivoMAbmouseIgG2aisotypecontrol,unknownspecificity |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | BALB.BMouselymphnodeandspleenTcells |
| ReportedApplications | Flowcytometry |
| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |
