The TY/23 monoclonal antibody reacts with mouse CD73 also known as ecto-5'-nucleotidase (5'-NT), a 69 kDa GPI-anchored cell-surface protein. In mice, expression of CD73 is restricted to CD11b+ myeloid cells in the bone marrow and T cells in the spleen. CD73 plays a strategic role in calibrating the duration, magnitude, and chemical nature of purinergic signals delivered to immune cells through the conversion of AMP to adenosine. This drives a shift from an ATP-driven proinflammatory environment to an anti-inflammatory milieu induced by adenosine. CD73 has been shown to be important for the immunosuppressive activity of regulatory T cells. The TY/23 antibody has been reported to inhibit the enzymatic activity of CD73.
| Isotype | Rat IgG2a, κ |
| Recomended Isotype Control(s) | InVivoMAb rat IgG2a isotype control, anti-trinitrophenol |
| Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | BALB/c mouse spleen cells and CHO cells transfected with the mouse CD73 gene |
| Reported Applications | in vivo CD73 blockade |
| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2 μM filtered |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein G |
| Storage | Undiluted at 4°C in the dark |
