The53-5.8monoclonalantibodyreactswithmouseCD8βalsoknownasLyt3.2.TheCD8antigenisatransmembraneglycoproteinthatactsasaco-receptorfortheTcellreceptor(TCR).LiketheTCR,CD8bindstoclassIMHCmoleculesdisplayedbyantigenpresentingcells(APC).CD8isprimarilyexpressedonthesurfaceofcytotoxicTcells,butcanalsobefoundonthymocytes,naturalkillercells,andsomedendriticcellsubsets.CD8mostcommonlyexistsasaheterodimercomposedofoneCD8αandoneCD8βchainhowever,itcanalsoexistasahomodimercomposedoftwoCD8αchains.BoththeCD8αandCD8βchainssharesignificanthomologytoimmunoglobulinvariablelightchains.ThemolecularweightofeachCD8chainisapproximately34kDa.The53-5.8antibodyhasbeenshowntodepleteCD8+TcellscompletelybutnotdepleteCD8+CD11c+dendriticcellswhenusedinvivo.
| Isotype | RatIgG1, κ |
| RecomendedIsotypeControl(s) | InVivoMAbratIgG1Isotypecontrol,anti-trinitrophenol InVivoMAbratIgG1isotypecontrol,anti-horserADIshperoxidase |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | Mousethymusorspleen |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |
