TheB133.5monoclonalantibodyreactswithhumanIFNγ(interferongamma)a20kDasolublepleiotropiccytokineandthesolememberofthetypeIIclassofinterferons.IFNγisprimarilyproducedbyactivatedlymphocytesincludingT,B,NKcells,andILCs.IFNγexertsimmunoregulatory,anti-proliferative,anti-viral,andproinflammatoryactivitiesandplaysanimportantroleinactivation,growth,anddifferentiationofTandBlymphocytes,macrophages,NKcellsandothernon-hematopoieticcelltypes.Additionally,IFNγinducestheproductionofcytokines,Fcreceptor,andadhesionmoleculesandup-regulatesMHCclassIandIIantigenexpressionbyantigenpresentingcellsduringanimmuneresponse.IFNγhasalsobeenshowntomodulatemacrophageeffectorfunctions,influenceisotypeswitchingandinducethesecretionofimmunoglobulinsbyBcells.IFNγsignalsthroughtheIFNgammareceptorwhichexistsasaheterodimercomposedofCD119(IFNgammareceptor1)andAF-1(IFNgammareceptor2).TheIFNγreceptorisexpressedubiquitouslyonalmostallcelltypeswiththeexceptionofmatureerythrocytes.TheB133.5antibodyisaneutralizingantibody.
| Isotype | MouseIgG1 |
| RecomendedIsotypeControl(s) | InVivoMAbmouseIgG1isotypecontrol,unknownspecificity |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | RecombinanthumanIFNγ |
| ReportedApplications | invitroIFNγneutralization |
| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |
