The528monoclonalantibodyreactswithanepitopeontheextracellulardomainofhumanEGFR(epidermalgrowthfactorreceptor)alsoknownasErbB-1.EGFRisa170kDacell-surfacereceptorandbelongstotheErbBfamilyofreceptors.EGFRsignalingisactivateduponbindingoneofitsligandsincludingepidermalgrowthfactor(EGF),transforminggrowthfactorα(TGFα),Amphiregulin,andheparinbinding-EGF(HB-EGF).Uponactivation,EGFRtransitionsfromaninactivemonomericformtoanactivehomodimer.ThisinitiatesseveraldownstreamsignaltransductioncascadesincludingtheMAPK,AktandJNKpathways,leADIngtoDNAsynthesisandcellproliferation.EGFRoverexpressionorconstitutiveactivationareassociatedwithmanycancers.Forthisreason,anti-EGFRmonoclonalantibodymediatedimmunotherapiesarecurrentlybeingexploredascancertreatments.The528antibodyhasbeenreportedtoblockEGFbindingtoitsreceptorandinhibitA431tumorformationinnudemice.
| Isotype | MouseIgG2a |
| RecomendedIsotypeControl(s) | InVivoMAbmouseIgG2aisotypecontrol,unknownspecificity |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | PurifiedEGFRfromA431cells |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |
