TheMP2-8F8monoclonalantibodyreactswithmouseIL-3,apleiotropiccytokineproducedprimarilybyactivatedTcellsbutalsobyactivatedmastcells,keratinocytesandneurons/astrocytes.IL-3ishighlyspecies-specific,maturehumanandmouseIL-3shareonly29%sequenceidentityandhumanIL-3doesnotshowactivityonmousecells.IL-3canstimulatetheproliferationanddifferentiationofmegakaryocytes,neutrophils,andmacrophagesfrombonemarrowcultures.Additionally,IL-3alsoaffectsthefunctionalactivityofmaturemastcells,basophils,eosinophilsandmacrophages.TheMP2-8F8antibodyhasbeenreportedtoneutralizethebioactivityofnaturalorrecombinantIL-3aswellasstimulatebasophilproliferationwhenadmiNISTeredasacomplexwithIL-3.
| Isotype | RatIgG1 |
| RecomendedIsotypeControl(s) | InVivoMAbratIgG1isotypecontrol,anti-horserADIshperoxidase InVivoMAbratIgG1Isotypecontrol,anti-trinitrophenol |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | COS-expressed,recombinantmouseIL-3 |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |
