ThePC-61.5.3monoclonalantibodyreactswithmouseIL-2RαalsoknownasCD25,Ly-43,p55,orTac.IL-2Rαisthe55kDaligand-bindingsubunitoftheinterleukin2receptoralphachain.IL-2RαisexpressedonactivatedmatureTandBlymphocytes,thymocytesubsets,pre-Bcells,andTregulatorycells.IL-2Rαhasbeenshowntoplayrolesinlymphocytedifferentiation,activation,andproliferation.Alone,theIL-2RαbindsIL-2withrelativelylowaffinityhowever,whenIL-2RαassociateswithIL-2Rβ(CD122)andthecommongammachain(CD132)thecomplexbindsIL-2withhighaffinity.ThePC-61.5.3antibodyhasbeenshowntoinhibitthebindingofIL-2toboththelowandhighaffinityIL-2receptorforms.Additionally,thePC-61.5.3antibodyiscommonlyusedtodepleteCD4+FoxP3+Tregulatorycellsinvivo.
Isotype | RatIgG1, λ |
RecomendedIsotypeControl(s) | InVivoPlusratIgG1isotypecontrol,anti-horserADIshperoxidase InVivoPlusratIgG1isotypecontrol,anti-trinitrophenol |
RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
Immunogen | IL-2-dependentcytolyticmouseTcellcloneB6.1 |
ReportedApplications |
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Endotoxin |
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Purity |
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Aggregation |
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Formulation |
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Sterility | 0.2μMfiltered |
Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
Purification | ProteinAHighSalt |
Storage | Undilutedat4°Cinthedark |
MurinePathogenTestResults |
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