TheJ43monoclonalantibodyreactswithmousePD-1(programmeddeath-1)alsoknownasCD279.PD-1isa50-55kDacellsurfacereceptorencodedbythePdcd1genethatbelongstotheCD28familyoftheimmunoglobulinsuperfamily.PD-1istransientlyexpressedonCD4andCD8thymocytesaswellasactivatedTandBlymphocytesandmyeloidcells.PD-1expressiondeclinesaftersuccessfuleliminationofantigen.Additionally,Pdcd1mRNAisexpressedindevelopingBlymphocytesduringthepro-B-cellstage.PD-1’sstructureincludesaITIM(immunoreceptortyrosine-basedinhibitorymotif)suggestingthatPD-1negativelyregulatesTCRsignals.PD-1signalsviabindingitstwoligands,PD-L1andPD-L2bothmembersoftheB7family.Uponligandbinding,PD-1signalinginhibitsT-cellactivation,leADIngtoreducedproliferation,cytokineproduction,andT-celldeath.Additionally,PD-1isknowntoplaykeyrolesinperipheraltoleranceandpreventionofautoimmunediseaseinmiceasPD-1knockoutanimalsshowdilatedcardiomyopathy,splenomegaly,andlossofperipheraltolerance.InducedPD-L1expressioniscommoninmanytumorsincludingsquamouscellcarcinoma,colonadenocarcinoma,andbreastadenocarcinoma.PD-L1overexpressionresultsinincreasedresistanceoftumorcellstoCD8Tcellmediatedlysis.Inmousemodelsofmelanoma,tumorgrowthcanbetransientlyarrestedviatreatmentwithantibodieswhichblocktheinteractionbetweenPD-L1anditsreceptorPD-1.Forthesereasonsanti-PD-1mediatedimmunotherapiesarecurrentlybeingexploredascancertreatments.TheJ43antibodyhasbeenshowntoblockthebindingofbothmousePD-L1-IgandmousePD-L2-IgtoPD-1.
| Isotype | ArmenianHamsterIgG |
| RecomendedIsotypeControl(s) | InVivoPluspolyclonalArmenianhamsterIgG |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH6.5DilutionBuffer |
| Immunogen | SyrianHamsterBKHcellstransfectedwithmousePD-1CDNA |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Aggregation |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |
| MurinePathogenTestResults |
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