TheDTA-1monoclonalantibodyreactswithmouseGITR(glucocorticoid-inducedTNFR-relatedgene),a66-70kDaco-stimulatoryimmunecheckpointmoleculebelongingtotheTumorNecrosisFactorsuperfamily(TNFRSF18).GITRisexpressedatlowlevelsonrestingTlymphocytesandathighlevelsonregulatoryTcells.GITRisupregulatedonactivatedTcellswhereitprovidesco-stimulation.GITRligand(GITRL)isfoundonBcells,macrophages,dendriticandendothelialcells,andisimplicatedinregulatingbothinnateandadaptiveimmuneresponses.GITRisalsothoughttoplayakeyroleindominantimmunologicalself-tolerancemaintainedbyregulatoryTcells.KnockoutstudiesinmicealsosuggesttheroleofthisreceptorisintheregulationofCD3-drivenTcellactivationandprogrammedcelldeath.TheDTA-1antibodyisanagoNISTicantibodythatiscommonlyusedtoinduceGITRsignalinginvivo.
| Isotype | RatIgG2b, λ |
| RecomendedIsotypeControl(s) | InVivoPlusratIgG2bisotypecontrol,anti-keyholelimpethemocyanin |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer |
| Immunogen | MouseCD25+ CD4+ Tcells |
| ReportedApplications | invivoGITRstimulation |
| Endotoxin |
|
| Purity |
|
| Aggregation |
|
| Formulation |
|
| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| Storage | Undilutedat4°Cinthedark |
| MurinePathogenTestResults |
|
