The RB6-8C5 monoclonal antibody reacts strongly with mouse Ly6G and weakly with mouse Ly6C previously referred to as GR-1. Ly6G is a 21-25 kDa member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Ly6G is expressed differentially during development by cells in the myeloid lineage including monocytes macrophages granulocytes and neutrophils. Monocytes typically express Ly6G transiently during development while mature granulocytes and peripheral neutrophils retain expression making Ly6G a good cell surface marker for these populations.The RB6-8C5 antibody has been shown to inhibit the binding of the 1A8 antibody. The 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross reactivity with Ly6C.
| Isotype | Rat IgG2b, κ |
| Recomended Isotype Control(s) | InVivoPlus rat IgG2b isotype control, anti-keyhole limpet hemocyanin |
| Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Mouse granulocytes |
| Reported Applications |
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| Endotoxin |
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| Purity |
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| Aggregation |
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| Formulation |
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| Sterility | 0.2 μM filtered |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein G |
| Storage | Undiluted at 4°C in the dark |
| Murine Pathogen Test Results |
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