The C9B7W monoclonal antibody reacts with mouse LAG-3 also known as CD223. LAG-3 is a 70 kDa type I transmembrane glycoprotein encoded by the Lag3 gene that belongs to the immunoglobulin superfamily. LAG-3 is expressed by activated T lymphocytes, NK cells, and T regulatory cells. LAG-3’s main ligand is MHC class II which it binds to with a higher affinity than even CD4 does. Upon binding LAG-3 is thought to play similar roles as CTLA-4 and PD-1 including downregulation of TCR signaling and inhibition of CD4-dependent T cell function. LAG-3 has also been demonstrated to contribute to the suppressor function of T regulatory cells. In contrast to inhibition, LAG-3 has been shown to promotes immune responses by activating antigen-presenting cells. The C9B7W antibody has been reported to block the function of murine LAG-3 in vivo and in vitro but studies suggest that the antibody does not block binding of LAG-3 to MHC class II.
| Isotype | Rat IgG1, κ |
| Recomended Isotype Control(s) |
InVivoPlus rat IgG1 isotype control, anti-horseradish peroxidase InVivoPlus rat IgG1 isotype control, anti-trinitrophenol |
| Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Mouse CD223-Ig fusion protein |
| Reported Applications |
|
| Endotoxin |
|
| Purity |
|
| Aggregation |
|
| Formulation |
|
| Sterility | 0.2 μM filtered |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein G |
| Storage | Undiluted at 4°C in the dark |
| Murine Pathogen Test Results |
|
