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当前位置: 首页 > 产品中心 > monoclonal_antibody > BioXcell/InVivoMAb anti-mouse CD3ε F(ab)2 fragment/DISCOUNTED ACADEMIC OR NON PROFIT: 5MG/BE0001-1FAB-A005mg
商品详细BioXcell/InVivoMAb anti-mouse CD3ε F(ab)2 fragment/DISCOUNTED ACADEMIC OR NON PROFIT: 5MG/BE0001-1FAB-A005mg
BioXcell/InVivoMAb anti-mouse CD3ε F(ab)2 fragment/DISCOUNTED ACADEMIC OR NON PROFIT: 5MG/BE0001-1FAB-A005mg
BioXcell/InVivoMAb anti-mouse CD3ε F(ab)2 fragment/DISCOUNTED ACADEMIC OR NON PROFIT: 5MG/BE0001-1FAB-A005mg
商品编号: BE0001-1FAB-A005mg
品牌: BioXcell inc
市场价: ¥10000.00
美元价: 6000.00
产地: 美国(厂家直采)
公司:
产品分类: 单克隆抗体
公司分类: monoclonal_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

About InVivoMAb anti-mouse CD3ε F(ab")2 fragment

The anti-mouse CD3 F(ab")2 fragment is the antigen binding F(ab")2 fragments of the 145-2C11 monoclonal antibody only. The majority of the Fc fragment has been removed via pepsin digestion. The 145-2C11 antibody F(ab")2 fragment reacts with mouse CD3ε, a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3ε is one of five polypeptide chains that combine to form the TCR complex. CD3ε is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The complete 145-2C11 antibody is commonly used to stimulate T cells in vitro however, when used in vivo activation of resting T cells results in cytokine release and toxicity caused by Ab-mediated cross-linking of T cells and Fcγ receptor-bearing cells. To avoid these complications non-Fc receptor binding F(ab’)2 fragments of the 145-2C11 antibody are commonly used. This non-FcR-binding anti-CD3 induces apoptosis of Ag-activated T cells in vivo by allowing durable expression of the TCR and sustained signaling. Foxp3+ Tregs have been shown to be resistant to CD3 antibody-mediated depletion.

InVivoMAb anti-mouse CD3ε F(ab")2 fragment Specifications

Isotype

Armenian Hamster IgG1

Recommended Isotype Control(s)InVivoMAb hamster IgG f(ab")2 fragments(BE0091-FAB)
Recommended InVivoPure Dilution BufferInVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

Mouse BM10-37 cytotoxic T cells

Reported Applications

in vivo T cell depletion

Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Pepsin Digest

Purification

Protein A

Storage

The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

RRID

AB_2687679

品牌介绍
Bio X Cell采用最先进的发酵技术;包括搅拌罐发酵罐和波包系统;我们的生产能力从5升到500升不等。Bio X Cell提供从现有杂交瘤细胞系产生的抗体的生产和纯化服务。这些杂交瘤通常在客户的实验室中开发或在公共领域可用。该服务包括在细胞培养物中发酵杂交瘤,通过离心收获细胞培养上清液以及通过30 kDa超滤浓缩。通过蛋白A或G亲和色谱进行纯化。将纯化的抗体透析到PBS中并过滤除菌。我们所有定制产品的内毒素水平<2EU / mg。我们还提供冻结细胞系,现场细胞系存储和MAP测试的额外费用。