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BioXcell/InVivoPlus抗鼠PD-1（CD279）/折扣学术或非营利性100MG/BP0146-A100mg
AboutInVivoPlusanti-mousePD-1(CD279)
TheRMP1-14monoclonalantibodyreactswithmousePD-1(programmeddeath-1)alsoknownasCD279.PD-1isa50-55kDacellsurfacereceptorencodedbythePdcd1genethatbelongstotheCD28familyoftheIgsuperfamily.PD-1istransientlyexpressedonCD4andCD8thymocytesaswellasactivatedTandBlymphocytesandmyeloidcells.PD-1expressiondeclinesaftersuccessfuleliminationofantigen.Additionally,Pdcd1mRNAisexpressedindevelopingBlymphocytesduringthepro-B-cellstage.PD-1’sstructureincludesaITIM(immunoreceptortyrosine-basedinhibitorymotif)suggestingthatPD-1negativelyregulatesTCRsignals.PD-1signalsviabindingitstwoligands,PD-L1andPD-L2bothmembersoftheB7family.Uponligandbinding,PD-1signalinginhibitsT-cellactivation,leADIngtoreducedproliferation,cytokineproduction,andT-celldeath.Additionally,PD-1isknowntoplaykeyrolesinperipheraltoleranceandpreventionofautoimmunediseaseinmiceasPD-1knockoutanimalsshowdilatedcardiomyopathy,splenomegaly,andlossofperipheraltolerance.InducedPD-L1expressioniscommoninmanytumorsincludingsquamouscellcarcinoma,colonadenocarcinoma,andbreastadenocarcinoma.PD-L1overexpressionresultsinincreasedresistanceoftumorcellstoCD8Tcellmediatedlysis.Inmousemodelsofmelanoma,tumorgrowthcanbetransientlyarrestedviatreatmentwithantibodieswhichblocktheinteractionbetweenPD-L1anditsreceptorPD-1.Forthesereasonsanti-PD-1mediatedimmunotherapiesarecurrentlybeingexploredascancertreatments.LiketheJ43antibodytheRMP1-14antibodyhasbeenshowntoblockthebindingofbothmousePD-L1-IgandmousePD-L2-IgtoPD-1.
InVivoPlusanti-mousePD-1(CD279)Specifications
| Isotype | RatIgG2a, κ |
| RecommendedIsotypeControl(s) | InVivoPlusratIgG2aisotypecontrol,anti-trinitrophenol(BP0089) |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer(IP0070) |
| Immunogen | SyrianHamsterBKHcellstransfectedwithmousePD-1CDNA |
| ReportedApplications |
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| Endotoxin |
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| BindingValidation | Determinedbyimmunoblot |
| Purity |
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| Aggregation |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinG |
| MolecularWeight | 150kDa |
| MurinePathogenTestResults |
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| RRID | AB_10949053 |
| Storage | Theantibodysolutionshouldbestoredatthestockconcentrationat4°C.Donotfreeze. |
