About InVivoPlus anti-mouse CD40L (CD154)
The MR-1 monoclonal antibody reacts with mouse CD154 also known as CD40 ligand. CD154 exists as a 39 kDa accessory molecule and belongs to the TNF superfamily of cytokines. CD154 is primarily expressed on the surface of activated CD4+ T lymphocytes but can also be expressed by platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, CD8+ T lymphocytes as well as non-hematopoietic cells including smooth muscle cells, endothelial cells, and epithelial cells. CD154 signals through CD40 and is thought to play a key role in T and B lymphocyte costimulation. The MR-1 monoclonal antibody has been reported to inhibit in vitro activation of B lymphocytes by blocking the binding of CD154 with CD40 on T helper cells as well as inhibit the formation of germinal centers and disrupt antigen-specific T cell responses. Additionally, the MR-1 antibody blocks interactions of T cells and antigen-presenting cells in vitro and blocks the development of experimental autoimmune disease in vivo.
InVivoPlus anti-mouse CD40L (CD154) Specifications
| Isotype | Armenian Hamster IgG |
| Recommended Isotype Control(s) | InVivoPlus polyclonal Armenian hamster IgG(BP0091) |
| Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer(IP0070) |
| Immunogen | Activated mouse Th1 clone D1.6 |
| Reported Applications |
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| Endotoxin |
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| Binding Validation | Determined by immunoblot |
| Purity |
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| Aggregation |
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| Formulation |
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| Sterility | 0.2 μM filtered |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Test Results |
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| RRID | AB_1107601 |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
