AboutInVivoMAbanti-mouseCD25(IL-2Rα)
ThePC-61.5.3monoclonalantibodyreactswithmouseIL-2RαalsoknownasCD25,Ly-43,p55,orTac.IL-2Rαisthe55kDaligand-bindingsubunitoftheinterleukin2receptoralphachain.IL-2RαisexpressedonactivatedmatureTandBlymphocytes,thymocytesubsets,pre-Bcells,andTregulatorycells.IL-2Rαhasbeenshowntoplayrolesinlymphocytedifferentiation,activation,andproliferation.Alone,theIL-2RαbindsIL-2withrelativelylowaffinityhowever,whenIL-2RαassociateswithIL-2Rβ(CD122)andthecommongammachain(CD132)thecomplexbindsIL-2withhighaffinity.ThePC-61.5.3antibodyhasbeenshowntoinhibitthebindingofIL-2toboththelowandhighaffinityIL-2receptorforms.Additionally,thePC-61.5.3antibodyiscommonlyusedtodepleteCD4+FoxP3+Tregulatorycellsinvivo.
InVivoMAbanti-mouseCD25(IL-2Rα)Specifications
| Isotype | RatIgG1, λ |
| RecommendedIsotypeControl(s) | InVivoMAbratIgG1isotypecontrol,anti-horserADIshperoxidase(BE0088) |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer(IP0070) |
| Immunogen | IL-2-dependentcytolyticmouseTcellcloneB6.1 |
| ReportedApplications |
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| Endotoxin |
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| Purity |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromtissueculturesupernatantinananimalfreefacility |
| Purification | ProteinAHighSalt |
| Storage | Theantibodysolutionshouldbestoredatthestockconcentrationat4°C.Donotfreeze. |
| RRID | AB_1107619 |
| MolecularWeight | 150kDa |
