About InVivoMAb anti-West Nile/dengue virus E protein
The E60 monoclonal antibody reacts with West Nile virus (WNV) and dengue virus (DENV) envelope (E) protein. WNV and DENV are small, enveloped, single-stranded RNA viruses of the family Flaviviridae. The viral envelope of flaviviruses consists of multiple copies of the 50-60 kDa E protein and the 8 kDa M (membrane) protein. X-ray crystallography studies have revealed that the ectodomain of each E protein is comprised of three structural domains: DI, DII and DIII, connected by flexible hinges. The tip of the DII domain contains a conserved region termed the “fusion loop,” which is required for membrane fusion of the viral envelope with the host cell membrane. The E60 antibody is a flavivirus cross-reactive neutralizing antibody that binds to an epitope in the fusion loop peptide of domain II on the E protein.
InVivoMAb anti-West Nile/dengue virus E protein Specifications
| Isotype | Mouse IgG2a, κ |
| Recommended Isotype Control(s) | InVivoMAb mouse IgG2a isotype control, unknown specificity(BE0085) |
| Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer(IP0070) |
| Immunogen | Purified soluble WNV E protein |
| Reported Applications |
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| Formulation |
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| Endotoxin |
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| Purity |
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| Sterility | 0.2 μM filtered |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| RRID | AB_2819052 |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
