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当前位置: 首页 > 产品中心 > monoclonal_antibody > BioXcell/InVivoMAb anti-mouse TIM-3 (CD366)/DISCOUNTED ACADEMIC OR NON PROFIT: 100MG/BE0275-A100mg
商品详细BioXcell/InVivoMAb anti-mouse TIM-3 (CD366)/DISCOUNTED ACADEMIC OR NON PROFIT: 100MG/BE0275-A100mg
BioXcell/InVivoMAb anti-mouse TIM-3 (CD366)/DISCOUNTED ACADEMIC OR NON PROFIT: 100MG/BE0275-A100mg
BioXcell/InVivoMAb anti-mouse TIM-3 (CD366)/DISCOUNTED ACADEMIC OR NON PROFIT: 100MG/BE0275-A100mg
商品编号: BE0275-A100mg
品牌: BioXcell inc
市场价: ¥44000.00
美元价: 26400.00
产地: 美国(厂家直采)
公司:
产品分类: 单克隆抗体
公司分类: monoclonal_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

About InVivoMAb anti-mouse TIM-3 (CD366)

The B8.2C12 monoclonal antibody reacts with mouse TIM-3 (T cell immunoglobulin and mucin domain-3) also known as CD366. This antibody binds to the BALB/c allele of TIM-3 while reactivity to the C57Bl/6 allele is significantly weaker. TIM-3 is a 60 kDa member of the TIM family of immune checkpoint receptors and exists as a type I transmembrane glycoprotein with a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. TIM-3 is specifically expressed at high levels on the surface of Th1 lymphocytes whereas Th2 lymphocytes express TIM-1 and TIM-2. TIM-3 activation occurs via binding to the cell-associated C-type lectin galectin-9. Upon binding TIM-3 induces apoptosis of Th1 cells. Inhibition of TIM-3 signaling in mice has been shown to exacerbate experimental autoimmune encephalomyelitis, promote IFNγ production and Th1 cell proliferation. Tim-3 has also been shown to be required for the induction of tolerance, as both TIM-3 knockout animals and mice treated with TIM-3-Ig fusion protein display defects in the induction of antigen-specific tolerance. Additionally, TIM-3 signaling is currently being explored as a cancer immunotherapy target as CD8 T cells which express both TIM-3 and PD-1 exhibit greater defects in both cell-cycle progression and effector cytokine production than cells that express PD-1 alone.

InVivoMAb anti-mouse TIM-3 (CD366) Specifications

Isotype

Rat IgG1, κ

Recommended Isotype Control(s)InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase(BE0088)
Recommended InVivoPure Dilution BufferInVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

Mouse Tim-3 protein/Freund adjuvant

Reported Applications
  • in vivo TIM-3 neutralization
  • in vitro TIM-3 blocking
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

RRID

AB_2687798

Molecular Weight

150 kDa

品牌介绍
Bio X Cell采用最先进的发酵技术;包括搅拌罐发酵罐和波包系统;我们的生产能力从5升到500升不等。Bio X Cell提供从现有杂交瘤细胞系产生的抗体的生产和纯化服务。这些杂交瘤通常在客户的实验室中开发或在公共领域可用。该服务包括在细胞培养物中发酵杂交瘤,通过离心收获细胞培养上清液以及通过30 kDa超滤浓缩。通过蛋白A或G亲和色谱进行纯化。将纯化的抗体透析到PBS中并过滤除菌。我们所有定制产品的内毒素水平<2EU / mg。我们还提供冻结细胞系,现场细胞系存储和MAP测试的额外费用。