About InVivoMAb anti-mouse BTLA (CD272)
The 6F7 monoclonal antibody reacts with mouse B- and T-lymphocyte attenuator (BTLA) also known as CD272. BTLA is an Ig superfamily member which is expressed on B cells, T cells, macrophages, dendritic cells, NK cells, and NKT cells. Like PD-1 and CTLA-4, BTLA interacts with a B7 homolog, B7-H4. However, unlike PD-1 and CTLA-4, BTLA displays T cell inhibition via interaction with tumor necrosis family receptors, not just the B7 family of cell surface receptors. BTLA is a ligand for herpes virus entry mediator (HVEM). BTLA-HVEM complexes have been shown to negatively regulate T cell immune responses. There are conflicting reports concerning the activity of the 6F7 antibody. In some cases, the antibody is reported to deplete BTLA+ B and CD4 T cells while in others the antibody is reported as having agonistic activity. See the references for details.
InVivoMAb anti-mouse BTLA (CD272) Specifications
| Isotype | Mouse IgG1, κ |
| Recommended Isotype Control(s) | InVivoMAb mouse IgG1 isotype control, unknown specificity(BE0083) |
| Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer(IP0070) |
| Immunogen | Ig domain of C57BL/6 mouse BTLA |
| Reported Applications |
*see description for details |
| Endotoxin | <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay |
| Purity | >95% Determined by SDS-PAGE |
| Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
| Sterility | 0.2 μM filtered |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein G |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| RRID | AB_2721033 |
| Molecular Weight | 150 kDa |
