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当前位置: 首页 > 产品中心 > Antibody_set > BioXcell/ReadyTag anti-6-His/折扣学术或非盈利:5MG/RT0266-A005mg
商品详细BioXcell/ReadyTag anti-6-His/折扣学术或非盈利:5MG/RT0266-A005mg
BioXcell/ReadyTag anti-6-His/折扣学术或非盈利:5MG/RT0266-A005mg
BioXcell/ReadyTag anti-6-His/折扣学术或非盈利:5MG/RT0266-A005mg
商品编号: RT0266-A005mg
品牌: BioXcell inc
市场价: ¥3000.00
美元价: 1800.00
产地: 美国(厂家直采)
公司:
产品分类: 抗体套装
公司分类: Antibody_set
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

About InVivoMAb anti-mouse/rat/rabbit TNFα

The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit TNFα (tumor necrosis factor-alpha) a multifunctional proinflammatory cytokine. TNFα exists as a soluble 17 kDa monomer, which forms homotrimers in circulation or as a 26 kDa membrane-bound form. TNFα belongs to the TNF superfamily of cytokines and signals through its two receptors, TNFR1 and TNFR2 which can be activated by both the soluble trimeric and membrane-bound and forms of TNFα. TNFα is primarily produced by macrophages in response to foreign antigens such as bacteria (lipopolysaccharides), viruses, and parasites as well as mitogens and other cytokines but can also be expressed by monocytes, neutrophils, NK cells, CD4 T cells and some specialized dendritic cells. TNFα is known to play key roles in a wide spectrum of biological processes including immunoregulation, cell proliferation, differentiation, apoptosis, antitumor activity, inflammation, anorexia, cachexia, septic shock, hematopoiesis, and viral replication. TNFα dysregulation has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Mouse and human TNFα share 79% amino acid sequence identity however, mouse TNFα is glycosylated while human TNFα is not. TNFα knockout animals display defects in response to bacterial infection, characterized by defects in forming organized follicular dendritic cell networks and germinal centers with a lack of primary B cell follicles. The TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-α.

InVivoMAb anti-mouse/rat/rabbit TNFα Specifications

Isotype

Armenian Hamster IgG, κ

Recommended Isotype Control(s)InVivoMAbpolyclonal Armenian hamster IgG(BE0091)
Recommended InVivoPure Dilution BufferInVivoPure pH 7.0 Dilution Buffer(IP0070)
Immunogen

Recombinant mouse TNFα

Reported Applications
  • in vivo TNFα neutralization
  • Flow cytometry
Endotoxin
  • <2EU/mg (<0.002EU/μg)
  • Determined by LAL gel clotting assay
Purity
  • >95%
  • Determined by SDS-PAGE
Formulation
  • PBS, pH 7.0
  • Contains no stabilizers or preservatives
Sterility

0.2 μM filtered

Production

Purified from tissue culture supernatant in an animal free facility

Purification

Protein G

Storage

The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

RRID

AB_2687725

Molecular Weight

150 kDa

品牌介绍
Bio X Cell采用最先进的发酵技术;包括搅拌罐发酵罐和波包系统;我们的生产能力从5升到500升不等。Bio X Cell提供从现有杂交瘤细胞系产生的抗体的生产和纯化服务。这些杂交瘤通常在客户的实验室中开发或在公共领域可用。该服务包括在细胞培养物中发酵杂交瘤,通过离心收获细胞培养上清液以及通过30 kDa超滤浓缩。通过蛋白A或G亲和色谱进行纯化。将纯化的抗体透析到PBS中并过滤除菌。我们所有定制产品的内毒素水平<2EU / mg。我们还提供冻结细胞系,现场细胞系存储和MAP测试的额外费用。